Dr. Bruno Le Pioufle - Microfluidics for the cell monitoring – from the single cell to cell tissue – energy and medical applications

Dr. Bruno Le Pioufle

Institut D’Alembert, ENS Paris-Saclay, Université Paris-Saclay

Microfluidics for the cell monitoring – from the single cell to cell tissue – energy and medical applications

The talk will firstly focus on how the microfluidic technology, combined to the integration of electrical functions, can be used for the sensing of single cells, for various applications.
Each cell has its own electrical signature, determined by its complex permittivity, depending from the electrical properties of its components (membranes, cytoplasm, intra cytoplasmic components, etc…).
I will remind how these properties can be determined, thanks to several methods like impedance spectroscopy, electrorotation, … Applications for cancer diagnosis, sickle cell disease, and microalgae lipid content will be shown.
Secondly I will focus on the use of microfluidics to pattern and culture cell tissues. The cases of liver-on chip, and study of liver-gut interaction will be discussed.

Selected publications

• R. Kida, A. Rajendran, M. Tsugane, J.-C. Duclos-Vallée, M. M. Mahe, S. Bensalem, H. Suzuki and B. Le Pioufle, Talanta Open, Gut-liver interaction study on an all-polydimethylsiloxane microfluidic device integrating intestinal paracellular permeability assay, 100289, 2024
• Y.-S. Lin, C.-l. Sun, S. Tsang, S. Bensalem, B. Le Pioufle and H.-Y. Wang, Biophysical Journal, Label-free and noninvasive analysis of microorganism surface epistructures at the single-cell level, 122, (10), 1794-1806, 2023
• Y.-S. Lin, S. Tsang, S. Bensalem, C.-C. Tsai, S.-J. Chen, C.-l. Sun, F. Lopes, B. Le Pioufle and H.-Y. Wang, Biosensors and Bioelectronics, Electrorotation of single microalgae cells during lipid accumulation for assessing cellular dielectric properties and total lipid contents, 173, 112772, 2021
• M. Boul, N. Benzoubir, A. Messina, R. Ghasemi, I. B. Mosbah, J.-C. Duclos-Vallée, A. Dubart-Kupperschmitt and B. Le Pioufle, Scientific Reports, A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation, 11, (1), 14075, 2021
• T. Xu, M. A. Lizarralde-Iragorri, J. Roman, R. Ghasemi, J.-P. Lefèvre, E. Martincic, V. Brousse, O. Français, W. El Nemer and B. Le Pioufle, Scientific Reports, Characterization of red blood cell microcirculatory parameters using a bioimpedance microfluidic device, 10, (1), 9869, 2020
• Y.-S. Lin, S. Tsang, S. Bensalem, C.-C. Tsai, S.-J. Chen, C.-l. Sun, F. Lopes, B. Le Pioufle and H.-Y. Wang, Biosensors and Bioelectronics, Electrorotation of single microalgae cells during lipid accumulation for assessing cellular dielectric properties and total lipid contents, 112772, 2020
• C. I. Trainito, D. C. Sweeney, J. Čemažar, E. M. Schmelz, O. Français, B. Le Pioufle and R. V. Davalos, Plos One, Characterization of sequentially-staged cancer cells using electrorotation, 14, (9), e0222289, 2019
• E. Bisceglia, M. Cubizolles, C. I. Trainito, J. Berthier, C. Pudda, O. Français, F. Mallard and B. Le Pioufle, Sensors and Actuators B: Chemical, A generic and label free method based on dielectrophoresis for the continuous separation of microorganism from whole blood samples, 212, 335-343, 2015